Journal: Atherosclerosis

Article Title: Identifying novel drug targets for calcific aortic valve disease through Mendelian randomization.

doi: 10.1016/j.atherosclerosis.2025.119110

Figure Lengend Snippet: Fig. 4. Cell-type specific expression in the aortic valve tissue for the proteins with PPH4 > 0.8. (A) Uniform manifold of approximation and projection clustering map of the aortic valves from 2 healthy donors and 4 patients with CAVD. (B–H) Expression patterns of ANGPTL4, PCSK9, ITGAV, CTSB, GNPTG, and FURIN in each aortic valve cell type. Red dots represent cells expressing the corresponding proteins. VIC, valvular interstitial cell; VDSC, valve-derived stromal cell; VEC, valvular endothelial cell. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: The primary antibodies used were as follows: ANGPTL4 (cat #18374-1-AP, Proteintech, 1:250); ITGAV (cat # 27096- 1-AP, Proteintech, 1:500).

Techniques: Expressing, Derivative Assay

Journal: Atherosclerosis

Article Title: Identifying novel drug targets for calcific aortic valve disease through Mendelian randomization.

doi: 10.1016/j.atherosclerosis.2025.119110

Figure Lengend Snippet: Fig. 5. Upregulation of ANGPTL4 and ITGAV in osteogenic VICs and calcific aortic valves. (A) Volcano plot showing differentially expressed genes between VICs cultured in the growth medium and the osteogenic medium. The x-axis represents the fold change, while the y-axis shows the q-value. (B) Representative WB image of ANGPTL4, ITGAV, and ALP in VICs. (C) Quantification of protein levels from WB analysis. (D) Representative immunofluorescence images showing ANGPTL4 and ITGAV expression in human aortic valves. (E) Quantification of mean fluorescence intensity for ANGPTL4 and ITGAV. **p < 0.01, ***p < 0.001 vs. GM or Non-CAVD. GM, growth medium; OM, osteogenic medium.

Article Snippet: The primary antibodies used were as follows: ANGPTL4 (cat #18374-1-AP, Proteintech, 1:250); ITGAV (cat # 27096- 1-AP, Proteintech, 1:500).

Techniques: Cell Culture, Immunofluorescence, Expressing, Fluorescence